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Fig. 2 | Cell & Bioscience

Fig. 2

From: Exosomes from hyperglycemia-stimulated vascular endothelial cells contain versican that regulate calcification/senescence in vascular smooth muscle cells

Fig. 2

The effects of HUVEC-Exos on VSMCs senescence/calcification. a VSMCs were co-cultured with NG/HG-HUVEC-Exos or supernatant isolated from HG stimulated HUVECs (HG-HUVEC-sup) for 10 days, calcification was measured by Alizarin Red Staining (400×). b VSMCs were co-cultured with NG/HG-HUVEC-Exos or HG-HUVEC-sup for 48 h, senescence was examined via SA-β-gal staining (400×). c The protein expression levels of ALP and p21 were assayed by Western blot. The ALP and p21 levels were quantified and normalised to GAPDH. Data are presented as the means ± SEM of three independent experiments. Statistical analysis was performed using one-way ANOVA test. **p < 0.01 compared with VSMCs group, HG-HUVEC-sup group and NG-HUVEC-Exos group

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