Table 1 Primers used in this study
From: Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro
Gene | Accession No. | Sense primer (5′–3′) | Antisense primer (5′–3′) | Amplicon length (bp) |
|---|---|---|---|---|
Semiquantitative qPCR | ||||
GAPDH | NM_002046 | AGTCAACGGATTTGGTCGTA | ACCATGTAGTTGAGGTCAATGAAG | 111 |
ST8SiaII | XM_017022642 | CCAGCTGTTGTTGACAGAAGTAA | TAAAATCTGCTTCCTGATCCTC | 111 |
ST8SiaIV | XM_011543630 | CTTCCAGCACAATGTAGAAGGTTG | GCTCTTGACCACTGACACATCTC | 112 |
Fluorescence-based RT-qPCR | ||||
GAPDH | NM_002046 | AAGATGCGGCTGACTGTCG | GTGACCAGGCGCCCAATAC | 113 |
ACTB | NM_001101 | TCCTGTGGCATCCACGAAACTA | CGGCAATGCCAGGGTACA | 121 |
EEF1A1 | NM_001402 | AAAATGGGAAAGGAAAAGACTCA | GGGGCATCAATGATAGTCACA | 281 |
ST8SiaII | XM_017022642 | GGAGGCAGAGGTACAATCAGATCA | TTCTGTCAACAACAGCTGGTGAT | 106 |
ST8SiaIV | XM_011543630 | TTGTCTTTGAGTCGGTCACTTGT | TCTAGGACCAAAGAGGAATTGATT | 116 |