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Fig. 3 | Cell & Bioscience

Fig. 3

From: Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro

Fig. 3

PolySia and cell migration. a HUVEC migration was examined by scratch assay in the absence (endoN treatment) or presence of polySia (no endoN). b Areas of untreated cell settings (control) were determined and set to 100%. Values are means of 18 scratches. The statistical evaluation was performed by paired Student’s t test (two tailed). Significance level is indicated by **p < 0.005. c The presence of polySia in the applied cell culture medium was assessed by immunostaining with anti-polySia antibody by Western blotting. Samples were pretreated with buffer (−) or endoN (+) before analysis

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