Fig. 3

PolySia and cell migration. a HUVEC migration was examined by scratch assay in the absence (endoN treatment) or presence of polySia (no endoN). b Areas of untreated cell settings (control) were determined and set to 100%. Values are means of 18 scratches. The statistical evaluation was performed by paired Student’s t test (two tailed). Significance level is indicated by **p < 0.005. c The presence of polySia in the applied cell culture medium was assessed by immunostaining with anti-polySia antibody by Western blotting. Samples were pretreated with buffer (−) or endoN (+) before analysis