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Fig. 5 | Cell & Bioscience

Fig. 5

From: TIPE2 acts as a biomarker for GIST risk category and suppresses the viability and invasiveness of GIST cells

Fig. 5

TIPE2 induced apoptosis and suppressed the expression of MMP9 in a Rac1-dependent manner. a Caspase-9/3 activity of GIST-T1 cells that transfected with TIPE2 overexpression plasmid were examined using the fluorescent caspase activity assay. b Caspase-9/3 activity of GIST-T1 cells that transfected with Rac1 siRNA were examined using the fluorescent caspase activity assay. c Caspase-9/3 activity of GIST-T1 cells that pretreated with NSC2366 were examined using the fluorescent caspase activity assay. d GIST-T1 cells that transfected with Mock, TIPE2 overexpression plasmid and TIPE2 mutant plasmid were applied to the fluorescent caspase activity assays. e The protein level of uPA, MMP-2 and MMP-9 were detected by ELISA in GIST-T1 cells transfected with TIPE2 overexpression plasmid. f The protein level of MMP-9 was detected by ELISA in GIST-T1 cells transfected with Rac1 siRNA. G. The protein level of MMP-9 was examined by ELISA in GIST-T1 cells pretreated with NSC23766. H. GIST-T1 cells that transfected with Mock, TIPE2 overexpression plasmid and TIPE2 mutant plasmid were used for MMP-9 activity assays. *P < 0.05. i Representative immunohistochemical staining showed high TIPE2 expression and low MMP-9 expression in low risk GIST tissues, while the MMP-9 expression was high in high risk GIST tissues. j IHC sum scores showed low MMP-9 expression and high TIPE2 expression in low risk GIST tissues, while high MMP-9 expression and low TIPE2 expression in high risk GIST tissues

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