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Fig. 3 | Cell & Bioscience

Fig. 3

From: TIPE2 acts as a biomarker for GIST risk category and suppresses the viability and invasiveness of GIST cells

Fig. 3

TIPE2 overexpression could suppress the proliferation, colony formation, migration and invasion of GIST-T1 cells by targeting Rac1. a Real-time PCR showed that TIPE2 expression was low in GIST-T1 cells and TIPE2 expression was up-regulated after transfection of TIPE2 overexpression plasmid. b CCK8 assays revealed that TIPE2 overexpression could suppress the viability of GIST-T1 cells. c After TIPE2 overexpression, colony formation assays were conducted to evaluated the colony formation capacity of GIST-T1 cells. d, e GIST-T1 cells transfected with the TIPE2 overexpression plasmid were used for Transwell migration and invasion assays. f Representative immunohistochemical staining showed that Rac1 expression was gradually up-regulated in accordance with GIST risk grades. g CCK8 assay was conducted to evaluate the viability of GIST-T1 cells after transfection with Rac1 siRNA. h CCK8 assay was conducted to evaluate the viability of GIST-T1 cells after treatment of NSC23766, a specific Rac1 inhibitor. i Transwell invasion assay revealed that Rac1 silencing decreased the invasiveness of GIST cells. j Transwell invasion assay was conducted to evaluate the invasiveness of GIST cells after treatment of NSC23766. **P < 0.01. Data represent the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001

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