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Fig. 2 | Cell & Bioscience

Fig. 2

From: (+)-JQ1 attenuated LPS-induced microglial inflammation via MAPK/NFκB signaling

Fig. 2

(+)-JQ1 reduces expressions of LPS/Aβ-induced proinflammatory cytokines in microglia. a BV2 cells were pretreated with (+)-JQ1 or the vehicle (DMSO) at the indicated dose, followed by LPS (100 ng/ml) stimulation for 4 h. b Alternatively, BV2 cells were pretreated with either (+)-JQ1 (400 nM) or DMSO, followed by LPS stimulation for the indicated period. After incubation, the cells were collected and lysed for quantitative analysis of transcription levels of IL-1β, IL-6 and TNFα by qRT-PCR. c, d BV2 cells were pretreated with (+)-JQ1, DMSO, or minomycine (mino) at the indicated dose, followed by LPS (100 ng/ml) or Aβ (10 μM) stimulation for 24 h. IL-1β, IL-6 and TNFα levels in the culture medium were determined via ELISA. The data are presented as the mean ± SEM (n = 3, * p ≤ 0.05)

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