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Fig. 3 | Cell & Bioscience

Fig. 3

From: CITED1 promotes proliferation of papillary thyroid cancer cells via the regulation of p21 and p27

Fig. 3

CITED1 promotes proliferation of PTC cell lines. a Overexpression of CITED1 was confirmed in the studied cells (TPC-1 and BCPAP) by western blotting analysis. α-tubulin was used as the loading control. b MTT assays were conducted to examine the viability of the indicated cell lines. c Representative images (upper panel) and relative quantification (lower panel) of the colony formation assay results. d Proportions of cells in distinct cell-cycle phases in the indicated cell lines were determined by flow cytometry. Overexpression of CITED1 decreased the proprotion of cells in G1 phase. e Representative micrographs (left panel) and quantification (right panel) of the EdU incorporation assay results. For b, c, and e, the results derived from three independent experiments and are expressed as the mean ± SD. *P < 0.05

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