Fig. 2

Silencing of CITED1 inhibits cell proliferation. a Protein expression of CITED1 in the indicated cells was analyzed by western blotting analysis. α-Tubulin was used as the loading control. b An MTT assay was performed to investigate the effect of CITED1 on the viability of the indicated PTC cells at the indicated time points. c Representative micrographs and relative quantification of colony formation assays of the indicated cells. d Flow-cytometric determination of the proportion of the studied cells in distinct cell-cycle phases. Knockdown of CITED1 increased the proportion of cells in G1 phase. e Representative images and relative quantification of EdU incorporation assays. For c and e, the data are reported as the mean ± SD of three independent experiments. *P < 0.05