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Fig. 2 | Cell & Bioscience

Fig. 2

From: Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing

Fig. 2

Diagram of the CRISPR/Cas9, CRISPR/Cas12a and CRISPR/Cas13 genome editing protocol. DRs (red) and spacer (blue) constitute the sequence of sgRNA (in Cas9 systems) or crRNA (in Cas12a and Cas13 systems). Optimal PAMs or PFSs highlighted in pink are critical for target recognition of these corresponding systems. In Cas9 staggered cleavage pattern, HNH cleaves TS to generate blunted end while RuvC cleaves NTS to generate non-blunted ends with 5′ 1- to 3-nt overhangs. The cleavage of Cas12a and Cas13 systems is in a staggered and collateral manner, respectively

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