Fig. 4

Overexpressed miR-29a-3p forced osteogenic differentiation and miR-29a-3p inhibitor reduced differentiation in vitro. BMSCs were transfected with miR-29a-3p enhancer, enhancer-nc, miR-29a-3p inhibitor, or inhibitor-nc, followed by the measurement of osteogenic differentiation by ALP staining (a), miR-29a-3p, ALP mRNA and Runx2 mRNA (b), and ALP and Runx2 proteins (c). Results are represented as mean ± SD. *P < 0.05