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Fig. 2 | Cell & Bioscience

Fig. 2

From: Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1

Fig. 2

PHLDA1 is regulated by miR-101 in gastric cancer cells. a PHLDA1 was a predicted as a target gene of miR-101 by TargetScan. b, c Luciferase assay was used to evaluate PHLDA1 3′UTR (wide typed or mutated one) activity in MKN-28 and HGC-27 cells respectively. d miR-101 decreased PHLDA1 mRNA expression in gastric cancer cells. MKN-28 and HGC-27 cells were transfected with miR-101 and then its expression was detected by qRT-PCR. e miR-101 decreased PHLDA1 protein expression in gastric cancer cells. MKN-28 and HGC-27 cells were transfected with miR-101 and then PHLDA1 protein level was examined by western blotting. **p < 0.01

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