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Fig. 1 | Cell & Bioscience

Fig. 1

From: A role of Pumilio 1 in mammalian oocyte maturation and maternal phase of embryogenesis

Fig. 1

Depletion of maternal Pum1 leads to abnormal preimplantation development. a The schematic of the four crosses used. Of note, zygotic Pum1 is not expressed maximally until four-cell stage, therefore maternal PUM1 protein is the only source of PUM1 protein during early preimplantation development. Cross I: m+z+ indicates presence of maternal and zygotic PUM1 protein, progeny are Pum1+/+; Cross II: m−z+ indicates maternal PUM1 absent and zygotic PUM1 present, progeny are Pum1+/; Cross III: m−z− indicates absence of both maternal and zygotic PUM1, progeny are Pum1/; Cross IV: m+z+ indicates maternal and zygotic PUM1 present from maternal Pum1 allele, progeny are Pum1/+. b, c Left panels show the percentages of embryos observed at each developmental stage (after excluding unfertilized oocytes). Right upper panels are representative light microscopy images of embryos collected at different time points. Scale bar (white): 500 µm. Right lower panel shows the number of matings for each cross and the mean number (± SD) of each type of embryo seen. Dpc: days postcoitum; 2C and 4C: two-cell and four-cell, embryos, respectively; 8C/M: eight-cell stage embryo and morula; B: blastocyst; abnormal: presumed fragmented embryos; *p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001

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