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Fig. 3 | Cell & Bioscience

Fig. 3

From: Inhibition of STAT3 in gastric cancer: role of pantoprazole as SHP-1 inducer

Fig. 3

Pantoprazole attenuates proliferation, migration, and invasion of gastric cancer cells. a WST-1 cell proliferation assay. All experiments were performed in triplicates. *P < 0.05, compared with control; #P < 0.05, compared with pantoprazole at 40 μg/ml (n = 3). b Matrigel invasion assay. Upper panel: representative images of invasion assay. Black bar indicates 50 μm. Lower panel: comparison of invading cells. Data are presented as mean ± standard deviation. Cell counting was performed in five randomly selected separate areas under ×20 magnification. *P < 0.05, compared with control; #P < 0.05, compared with pantoprazole at 40 μg/ml (n = 5). c Wound closure assay. Left panel: representative images of assay. Black bar indicates 500 μm. Right panel: Comparison of vertical wound distance. Data are presented as mean ± standard deviation. All experiments were performed in triplicates. *P < 0.05, compared with control; #P < 0.05, compared with pantoprazole at 40 μg/ml (n = 3). d 3-D culture spheroid cell invasion assay. Upper panel: representative images of 3-D culture spheroid invasion assay. Black bar indicates 200 μm. Lower panel: comparison of area of spheroids. Data are presented as mean ± standard deviation. All experiments were performed in triplicates. *P < 0.05, compared with control; #P < 0.05, compared with pantoprazole at 40 μg/ml (n = 3). PPZ, pantoprazole

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