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Table 1 Primer pairs used for qPCR

From: All-trans retinoic-acid inhibits heterodimeric bone morphogenetic protein 2/7-stimulated osteoclastogenesis, and resorption activity

Gene Acc. no Primer sequence Size (bp)
Ctsk NM_007802.3 F: 5′-CAGCAGAACGGAGGCATTGA-3′ R: 5′-CTTTGCCGTGGCGTTATACATACA-3′ 84
Acp5 NM_001102405.1 F: 5′-CCAATGCCAAAGAGATCGCC -3′ R: 5′-TCTGTGCAGAGACGTTGCCAAG -3′ 216
Calcr NM_007588.2 F: 5′-TTACCGACGAGCAACGCCTAC-3′ R: 5′-AGCAAGTGGGTTTCTGCACTCA-3′ 136
Rank AF_019046.1 F: 5′-CACACCCAGGACTATCATCAT-3′ R: 5′-CCCACCAAAGCATCTTCTGA-3′ 133
Nfatc1 EU_887572.1 F: 5′-TGGTGGCTTACCTTTCCCAA-3′ R: 5′-GCTCTTCACAGTCGTGCGAA-3′ 81
Rarα NM_001177302 F: 5′-AGCAGGCTCTACCTTGCCCT-3′ R: 5′-CAGTGGAAACCCAGCAGGAA-3′ 84
Rarβ NM_001042727 F: 5′-AAGTTAGTCTGCCGTCTGGAC-3′ R: 5′-TTGCCCATACCTTCAAGCAT-3′ 99
Rarγ NM_001042727 F: 5′-TGTGTTCATCCCTGTCCTGT-3′ R: 5′-CGAAAGGCAGTGCTGAGATT-3′ 128
c-Fos NM_010234.2 F: 5′-TGAGCAGTCAGAGAAGGCAA-3′ R: 5′-TTCACGAACAGGTAAGGTCCT-3′ 133
DC-Stamp NM_001347395.1  F: 5′-CCTGCTGCTCACAGATGGT-3′ R: 5′-TGGTGGTTGATGTTGGGAT-3′ 128
Gapdh NM_001289726.1 F: 5′-ACCACAGTCCATGCCATCAC-3′ R: 5′-TCCACCACCCTGTTGCTGTA-3′ 452
  1. Ctsk: cathepsin K, Acp5: acid phosphatase 5 tartrate resistant, Calcr: calcitonin receptor, Nfatc1: nuclear factor of activated T-cells cytoplasmic 1, Rar: retinoic acid receptor, Gapdh: aldehyde-3-phosphate dehydrogenase