Table 1 Primer pairs used for qPCR
Gene | Acc. no | Primer sequence | Size (bp) |
|---|---|---|---|
Ctsk | NM_007802.3 | F: 5′-CAGCAGAACGGAGGCATTGA-3′ R: 5′-CTTTGCCGTGGCGTTATACATACA-3′ | 84 |
Acp5 | NM_001102405.1 | F: 5′-CCAATGCCAAAGAGATCGCC -3′ R: 5′-TCTGTGCAGAGACGTTGCCAAG -3′ | 216 |
Calcr | NM_007588.2 | F: 5′-TTACCGACGAGCAACGCCTAC-3′ R: 5′-AGCAAGTGGGTTTCTGCACTCA-3′ | 136 |
Rank | AF_019046.1 | F: 5′-CACACCCAGGACTATCATCAT-3′ R: 5′-CCCACCAAAGCATCTTCTGA-3′ | 133 |
Nfatc1 | EU_887572.1 | F: 5′-TGGTGGCTTACCTTTCCCAA-3′ R: 5′-GCTCTTCACAGTCGTGCGAA-3′ | 81 |
Rarα | NM_001177302 | F: 5′-AGCAGGCTCTACCTTGCCCT-3′ R: 5′-CAGTGGAAACCCAGCAGGAA-3′ | 84 |
Rarβ | NM_001042727 | F: 5′-AAGTTAGTCTGCCGTCTGGAC-3′ R: 5′-TTGCCCATACCTTCAAGCAT-3′ | 99 |
Rarγ | NM_001042727 | F: 5′-TGTGTTCATCCCTGTCCTGT-3′ R: 5′-CGAAAGGCAGTGCTGAGATT-3′ | 128 |
c-Fos | NM_010234.2 | F: 5′-TGAGCAGTCAGAGAAGGCAA-3′ R: 5′-TTCACGAACAGGTAAGGTCCT-3′ | 133 |
DC-Stamp | NM_001347395.1 | F: 5′-CCTGCTGCTCACAGATGGT-3′ R: 5′-TGGTGGTTGATGTTGGGAT-3′ | 128 |
Gapdh | NM_001289726.1 | F: 5′-ACCACAGTCCATGCCATCAC-3′ R: 5′-TCCACCACCCTGTTGCTGTA-3′ | 452 |