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Fig. 4 | Cell & Bioscience

Fig. 4

From: Proteomic characterization of endogenous substrates of mammalian ubiquitin ligase Hrd1

Fig. 4

Accumulation of OS9 in Hrd1 and SEL1L knockout cells. a, b The level of endogenous OS9 was determined by immunoblotting using cell extracts from control or Hrd1 CRISPR null cells. The graph in b shows quantification from 3 independent experiments (error bars, sem, n = 3). c Overexpression of wild type Hrd1 reduces the level of OS9 in Hrd1 null cells. Where indicated, wild type (WT) or the C291A Hrd1 mutant were transfected into Hrd1 knockout cells at two different doses. Cell extracts were analyzed by immunoblotting. LE, long exposure; SE, short exposure; LC, loading control. d Cell extracts from control and three clones of SEL1L CRISPR null HEK293 cells were analyzed by immunoblotting with antibodies against the indicated proteins

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