Role of MCM2–7 protein phosphorylation in human cancer cells

Fei, Liangru; Xu, Hongtao

doi:10.1186/s13578-018-0242-2

Cell & Bioscience

Table 1 Summary of MCM phosphorylation in human cancer cells

From: Role of MCM2–7 protein phosphorylation in human cancer cells

Protein	Kinase	Phosphorylation site or domain	Biological and functional significance	References
MCM2	Cdc7	S27, S41, S139	Suggested to be essential for initiation of DNA replication and ATPase activity of MCM complex	[16]
	Cdc7	S5	Promotes pre-RC assembly during cell cycle re-entry	[37]
	Cdc7	S40, S53, S108	Regulates MCM2’s chromatin loading in a site-dependent manner	[19]
	Cdc7	S40, S108	Unclear	[43]
MCM4	Cdc7	N-terminal	Promotes MCM4’s interaction with Cdc45 on chromatin. Suggested to be essential for initiation of DNA replication and cell growth	[17]
MCM3	Cdk2/CycE	T722	Promotes MCM3’s chromatin loading. Regulates S phase checkpoint activation	[12]
MCM4	Cdk2	S3, T7, S32, S54, T110	Decreases chromatin loading of MCM complex to avoid re-replication during mitosis	[45]
MCM4	Cdk1, Cdk2	S3, T7, T19, S32, S54, S88, T110	Inhibits chromatin loading and helicase activity of MCM complex. Blocks DNA replication through inactivation of MCM complex	[20, 46,47,48,49, 51]
MCM7	Cdk2/CycE, Cdk1/CycB	S121	Promotes MCM complex formation. Regulates S checkpoint activation and mitotic exit	[13]
MCM2	ATR	S108	Responds to replication stress and stabilizes replication forks	[39]
MCM2	ATR	S108	Responds to replication stress	[38]
MCM3	ATM	S535	Responds to replication stress	[38]
MCM3	ATM, ATR	S728	Responds to replication stress	[58]
MCM6	ATR	S13	Responds to replication stress	[59]
MCM2	SIK1	Unclear	Activates helicase activity of MCM complex during DNA replication	[61]
MCM3	DAPK	S160	Unclear	[62]
MCM4	EBV-PK	T19, T110	Blocks DNA replication through inactivation of DNA unwinding by the MCM4/6/7 complex. Leads to cell growth arrest	[26]
MCM7	p56^Lyn	Tyr600	Promotes MCM complex formation, chromatin loading, DNA synthesis and cancer cell proliferation. Pathologically correlates with poor survival of breast cancer patients	[23]
	Akt	Unclear	Increases MCM7’s chromatin loading and MCM complex formation. Promotes DNA replication and cancer cell proliferation	[24]
	ILK	Unclear	Inhibits MCM7’s chromatin loading and cancer cell growth	[25]

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ISSN: 2045-3701

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