Skip to main content

Advertisement

Table 1 Summary of MCM phosphorylation in human cancer cells

From: Role of MCM2–7 protein phosphorylation in human cancer cells

Protein Kinase Phosphorylation site or domain Biological and functional significance References
MCM2 Cdc7 S27, S41, S139 Suggested to be essential for initiation of DNA replication and ATPase activity of MCM complex [16]
Cdc7 S5 Promotes pre-RC assembly during cell cycle re-entry [37]
Cdc7 S40, S53, S108 Regulates MCM2’s chromatin loading in a site-dependent manner [19]
Cdc7 S40, S108 Unclear [43]
MCM4 Cdc7 N-terminal Promotes MCM4’s interaction with Cdc45 on chromatin. Suggested to be essential for initiation of DNA replication and cell growth [17]
MCM3 Cdk2/CycE T722 Promotes MCM3’s chromatin loading. Regulates S phase checkpoint activation [12]
MCM4 Cdk2 S3, T7, S32, S54, T110 Decreases chromatin loading of MCM complex to avoid re-replication during mitosis [45]
Cdk1, Cdk2 S3, T7, T19, S32, S54, S88, T110 Inhibits chromatin loading and helicase activity of MCM complex. Blocks DNA replication through inactivation of MCM complex [20, 46,47,48,49, 51]
MCM7 Cdk2/CycE, Cdk1/CycB S121 Promotes MCM complex formation. Regulates S checkpoint activation and mitotic exit [13]
MCM2 ATR S108 Responds to replication stress and stabilizes replication forks [39]
ATR S108 Responds to replication stress [38]
MCM3 ATM S535 Responds to replication stress [38]
ATM, ATR S728 Responds to replication stress [58]
MCM6 ATR S13 Responds to replication stress [59]
MCM2 SIK1 Unclear Activates helicase activity of MCM complex during DNA replication [61]
MCM3 DAPK S160 Unclear [62]
MCM4 EBV-PK T19, T110 Blocks DNA replication through inactivation of DNA unwinding by the MCM4/6/7 complex. Leads to cell growth arrest [26]
MCM7 p56Lyn Tyr600 Promotes MCM complex formation, chromatin loading, DNA synthesis and cancer cell proliferation. Pathologically correlates with poor survival of breast cancer patients [23]
Akt Unclear Increases MCM7’s chromatin loading and MCM complex formation. Promotes DNA replication and cancer cell proliferation [24]
ILK Unclear Inhibits MCM7’s chromatin loading and cancer cell growth [25]