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Fig. 2 | Cell & Bioscience

Fig. 2

From: Long non-coding RNA CASC15 promotes melanoma progression by epigenetically regulating PDCD4

Fig. 2

Knockdown of CASC15 suppresses proliferation, enhanced apoptosis and decreased invasion in melanoma cells. A375 and M21 cells were transfected with si-con, si-CASC15 #1, or si-CASC15 #2. a, b qRT-PCR analysis was used to determine the relative expression of CASC15 in transfected A375 and M21 cells. c, d CCK-8 analysis was performed to measure the proliferation ability in transfected A375 and M21 cells. e, f Flow cytometry analysis was applied to detect cell cycle distribution in transfected A375 and M21 cells. The bar chart represents the percentage of cells in G1/G0, S, and G2/M phases. g, h The apoptotic rate of transfected A375 and M21 cells was analyzed by flow cytometry. i, j Transwell invasion assay was conducted to assess the invasive capability of transfected A375 and M21 cells. Results are represented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01

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