Fig. 4

Notch signaling plays the same function in the BMP4 model as in vivo. a FACS was used to analyze the optimal time and efficiency of PGCs and SSCs induced by different BMP4 concentrations. PGCs appeared on the day 4, and SSCs appeared on day 14 when treated with 40 ng/mL BMP4. b The overexpression of NICD in the in vitro model promoted the formation of PGCs and inhibited the formation of SSCs. Inhibition of NICD yielded the opposite results in this model. Scale bar: 50 µm. c, d Indirect immunofluorescence was used to detect the formation efficiency of PGCs and SSCs after NICD overexpression and inhibition. Scale bar: 50 µm