Fig. 3

Notch signalingpositively regulates the formation of PGCs and negatively regulates the formation of SSCs in vivo. a, b The expression levels of PGC and SSC marker genes were detected by qRT-PCR after NICD overexpression and inhibition in the in vitro model. c PGCs and SSCs formation efficiency was analyzed by flow cytometry after NICD overexpression and inhibition. d PAS staining of PGCs and SSCs following NICD overexpression and inhibition. PAS staining showed that NICD promoted the formation of PGCs, whereas inhibition of NICD expression inhibited the formation of PGCs. Arrow represents PGCs. Scale bar: 100 μm