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Fig. 2 | Cell & Bioscience

Fig. 2

From: NICD-mediated notch transduction regulates the different fate of chicken primordial germ cells and spermatogonial stem cells

Fig. 2

Notch signaling is dependent on CBF-1/RBP and regulates downstream gene expression. a The expression of MAML1, MAML2, and MAML3 genes was detected by qRT-PCR. b The expression of PCAF was detected by qRT-PCR. c Changes in HDAC1, HDAC2, and MAML1 levels in the CBF-1/RBP complex were detected by anti-RBP immunoprecipitation in PGCs with either Notch1 overexpression or inhibition. Β-actin served as the internal reference. d Anti-RBP immunoprecipitation was used to detect the changes of HDAC1 and HDAC2 in CBF-1/RBP complex after Notch1 overexpression and inhibition in SSCs. e The expression of HES1 was detected by qRT-PCR. f Colorimetric results showed that HDACs had significantly lower enzyme activity than SSCs in PGCs; overexpression of NICD could reduce HDACs enzyme activity, while inhibiting NICD could increase HDACs enzyme activity

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