Table 2 Known miRNAs identified in four life stages libraries of An. sinensis
miRNA name | Sequences | Length | Location | Tags per million (TPM) | |||
|---|---|---|---|---|---|---|---|
ASE | ASL | ASP | ASA | ||||
asi-miR-276-3p | UAGGAACUUCAUACCGUGCUC | 21 | KE525297.1:1458670..1458650:+ | 167,805.76 | 318,655.14 | 451,492.06 | 316,672.78 |
asi-miR-9a | UCUUUGGUUAUCUAGCUGUAUGA | 23 | KE525001.1:301001..301023:− | 141,229.57 | 137,568.26 | 57,223.48 | 27,174.76 |
asi-miR-1 | UGGAAUGUAAAGAAGUAUGGAG | 22 | KE524975.1:1035748..1035769:− | 187,484.83 | 88,731.09 | 100,424.03 | 115,685.16 |
asi-miR-100 | AACCCGUAGAUCCGAACUUGUG | 22 | KE525346.1:1178073..1178052+ | 5057.84 | 38,962.34 | 97,988.23 | 78,039.03 |
asi-miR-8-3p | UAAUACUGUCAGGUAAAGAUGUC | 23 | KE525347.1:277471..277449:+ | 2933.19 | 35,223.24 | 16,842.53 | 56,364.03 |
asi-miR-999 | UGUUAACUGUAAGACUGUGUCU | 22 | KE525161.1:40638..40659:- | 17,377.27 | 34,087.38 | 38,041.81 | 55,432.34 |
asi-miR-11-3p | CAUCACAGUCUGAGUUCUUGCU | 22 | KE525297.1:1145644..1145665− | 21,936.31 | 33,023.90 | 11,854.78 | 18,914.99 |
asi-miR-184 | UGGACGGAGAACUGAUAAGGGC | 22 | KE525421.1:213400..213421:− | 27,788.31 | 26,840.31 | 15,801.40 | 19,820.19 |
asi-miR-7 | UGGAAGACUAGUGAUUUUGUUGU | 23 | KE525340.1:256575..256553:+ | 69,849.63 | 25,689.28 | 18,252.89 | 35,200.06 |
asi-miR-281-3p | UGUCAUGGAAUUGCUCUCUUUA | 22 | KE525349.1:2646148..2646169:− | 5649.35 | 25,360.08 | 6667.99 | 34,188.91 |
asi-miR-9c-5p | UCUUUGGUAUUCUAGCUGUAGA | 22 | KE525317.1:660185..660164:+ | 25,959.34 | 23,472.43 | 13,744.82 | 18,872.14 |
asi-miR-263a-5p | AAUGGCACUGGAAGAAUUCACGG | 23 | KE525098.1:220160..220138:+ | 53,603.64 | 19,911.93 | 33,053.10 | 12,860.57 |
asi-miR-306 | UCAGGUACUGGAUGACUCUCAG | 22 | KE525317.1:662779..662758:+ | 26,686.89 | 17,783.81 | 6780.18 | 12,437.57 |
asi-miR-305-5p | AUUGUACUUCAUCAGGUGCUCUGG | 24 | KE524841.1:15363..15386:− | 4162.66 | 17,523.48 | 19,993.02 | 11,341.51 |
asi-miR-9b | ACUUUGGUGAUUUUAGCUGUAUG | 23 | KE525317.1:663617..663595:+ | 13,191.15 | 13,483.19 | 3319.73 | 10,659.89 |
asi-miR-10 | ACCCUGUAGAUCCGAAUUUGUU | 22 | KE525233.1:711575..711554:+ | 75,624.85 | 12,681.21 | 8573.87 | 8382.08 |
asi-miR-1174 | UCAGAUCUACUUCAUACCCAUG | 22 | KE525248.1:186355..186334+ | 5123.51 | 11,635.24 | 1359.33 | 7155.15 |
asi-miR-279 | UGACUAGAUCCACACUCAUUAA | 22 | KE525236.1:156609..156588+ | 7462.21 | 10,187.69 | 8815.69 | 7220.59 |
asi-miR-281-5p | AAGAGAGCUAUCCGUCGAC | 19 | KE525349.1:2646188..2646206:− | 1793.94 | 8863.89 | 2189.07 | 10,265.71 |
asi-miR-278-3p | UCGGUGGGACUUUCGUCCGUUU | 22 | KE525347.1:4349292..4349271:+ | 579.48 | 8851.05 | 2287.00 | 2259.11 |
asi-miR-13-3p | UAUCACAGCCAUUUUGACGAGUU | 23 | KE525350.1:1783388..1783366+ | 9781.06 | 7638.14 | 4976.66 | 4480.83 |
asi-miR-275-3p | UCAGGUACCUGAAGUAGCGC | 20 | KE524841.1:27273..27292:− | 511.15 | 6568.82 | 5975.96 | 5463.93 |
asi-let-7 | UGAGGUAGUUGGUUGUAUAGU | 21 | KE525346.1:11781486..1181466:+ | 61.76 | 6319.01 | 12,631.90 | 15,097.09 |
asi-miR-1175-5p | AAGUGGAGUAGUGGUCUCAUCG | 22 | KE525248.1:186488..1864678:+ | 1350.50 | 5971.13 | 98.09 | 1610.20 |
asi-miR-2765 | UGGUAACUCCACCACCGUUGGC | 22 | KE524732.1:275289..27549:− | 35.18 | 5915.09 | 37.08 | 14.02 |
asi-miR-2c | UAUCACAGCCAGCUUUGAUGAGC | 23 | KE525350.1:1783822..1783800+ | 5517.23 | 5218.17 | 4288.76 | 2824.67 |
asi-miR-2b | UAUCACAGCCAGCUUUGAUGAGCU | 24 | KE525350.1:1783823..1783800+ | 5510.66 | 5214.67 | 4290.50 | 2825.45 |
asi-miR-2a-3p | UAUCACAGCCAGCUUUGAAGAGC | 23 | KE525350.1:1783565:1783543:+ | 3337.86 | 4938.00 | 2844.96 | 2808.31 |
asi-miR-14 | UCAGUCUUUUUCUCUCUCCUAU | 22 | KE525231.1:338814..338835:− | 4248.19 | 4844.61 | 9771.57 | 11,007.32 |
asi-miR-34-5p | UGGCAGUGUGGUUAGCUGGUUG | 22 | KL645799.1:184..163:+ | 35.18 | 4643.82 | 102.85 | 16,234.44 |
asi-miR-375 | UUUGUUCGUUUGGCUCGAGUUA | 22 | KE525304.1.363119..363140:− | 2327.29 | 2722.32 | 7.45 | 25.71 |
asi-miR-8-5p | CAUCUUACCGGGCAGCAUUAGA | 22 | KE525347.1:277430..277409:+ | 1309.69 | 2236.69 | 1534.12 | 4757.38 |
asi-miR-1175-3p | UGAGAUUCUACUUCUCCGACUUAA | 24 | KE525248.1:186528..186505:+ | 50.35 | 2225.02 | 88.11 | 925.46 |
asi-miR-315-5p | UUUUGAUUGUUGCUCAGAAAGC | 22 | KE524190.1:262690..262711:− | 12,578.06 | 2041.74 | 778.55 | 765.76 |
asi-miR-252-5p | UAAGUACUAGUGCCGCAGGAG | 21 | KE525267.1:347072..347092:− | 2708.81 | 1847.96 | 1507.97 | 2164.85 |
asi-miR-317 | UGAACACAUCUGGUGGUAUCUCAGU | 25 | KE524744.1:7138..7162:− | 7.04 | 1746.39 | 127.72 | 2322.21 |
asi-miR-190 | AGAUAUGUUUGAUAUUCUUGGUUG | 24 | KE525352.1:3462214..3462237:− | 2311.34 | 1737.06 | 1090.57 | 2010.61 |
asi-miR-283 | UAAAUAUCAGCUGGUAAUUCU | 21 | KE524901.1:23378..23398:− | 2555.73 | 1557.28 | 621.19 | 1756.65 |
asi-miR-957 | UGAAACCGUCCAAAACUGAGGC | 22 | KE525321.1:54139..54160:− | 237.51 | 1363.50 | 1523.98 | 1921.02 |
asi-miR-998 | UAGCACCAUGAGAUUCAGC | 19 | KE524842.1:1145486..1145504:+ | 621.23 | 1236.25 | 352.11 | 509.47 |
asi-miR-263b-5p | CUUGGCACUGGGAGAAUUCACAG | 23 | KE524589.1:97550..97528:+ | 3141.31 | 1188.39 | 2647.35 | 1553.33 |
asi-miR-71-5p | AGAAAGACAUGGGUAGUGAGAU | 22 | KE525350.1:1781736..1781715:+ | 220.47 | 968.92 | 375.73 | 419.88 |
asi-miR-92b-3p | AAUUGCACUUGUCCCGGCCUGC | 22 | KE523980.1:64015..64036:+ | 647.34 | 938.57 | 175.74 | 151.91 |
asi-miR-277-3p | UAAAUGCACUAUCUGGUACGAC | 22 | KE524932.1:25145..25166:– | 891.26 | 797.32 | 15455.79 | 23,261.05 |
asi-miR-71-3p | UCUCACUACCUUGUCUUUCAUG | 22 | KE525350.1:1781809..1781788:+ | 346.19 | 652.56 | 309.17 | 257.85 |
asi-miR-125-5p | UCCCUGAGACCCUAACUUGUGA | 22 | KE525346.1:1182133..1182112:+ | 9.69 | 644.39 | 1732.05 | 1625.78 |
asi-miR-970 | UCAUAAGACACACGCGGCUAU | 21 | KE525003.1:917073..917053:+ | 603.56 | 630.38 | 650.98 | 843.66 |
asi-miR-981 | UUCGUUGUCGACGAAACCUGCA | 22 | KE524855.1:1042040..1042019:+ | 686.74 | 611.71 | 1152.22 | 895.08 |
asi-miR-31 | UGGCAAGAUGUUGGCAUAGCUGA | 23 | KE524855.1:565934..565957:− | 178.72 | 584.86 | 403.62 | 531.28 |
asi-miR-iab-4-5p | ACGUAUACUGAAUGUAUCCUGA | 22 | KE525233.1:295585..295606:− | 3101.28 | 573.18 | 135.96 | 222.80 |
asi-miR-12-5p | UGAGUAUUACAUCAGGUACUGGU | 23 | KE524901.1:21292..21314:+ | 512.40 | 554.50 | 502.18 | 771.99 |
asi-miR-92a-3p | UAUUGCACUUGUCCCGGCCUA | 21 | KL646680.1:1643..1623:+ | 572.28 | 552.17 | 152.45 | 129.31 |
asi-miR-9c-3p | UAAAGCUUUAGUACCAGAGGUC | 22 | KE525317.1:660231..660210:+ | 383.40 | 551.00 | 248.64 | 310.82 |
asi-miR-133 | UUGGUCCCCUUCAACCAGCUGU | 22 | KE524620.1:429840..429861:− | 264.41 | 441.27 | 500.12 | 662.93 |
asi-miR-305-3p | CGGCACAUGUUGGAGUACACUUA | 23 | KE524841.1:15325..15347:− | 160.90 | 429.59 | 460.98 | 166.71 |
asi-miR-282-5p | AAUCUAGCCUCUCCUAGGCUUUGUCUG | 27 | KE525264.1:323815..323789:+ | 1.25 | 391.07 | 73.37 | 52.19 |
asi-miR-996 | UGACUAGAUUACAUGCUCGUC | 21 | KE525286.1:157381..157361:+ | 107.26 | 347.88 | 231.05 | 482.98 |
asi-miR-87 | GGUGAGCAAAUAUUCAGGUGU | 21 | KE524974.1:1235367..1235347:+ | 320.39 | 326.87 | 406.47 | 736.16 |
asi-miR-993 | UACCCUGUAGUUCCGGGCUUUU | 22 | KE525233.1:748304..748325:− | 3470.14 | 261.49 | 182.08 | 255.51 |
asi-miR-989 | UGUGAUGUGACGUAGUGGUAC | 21 | KE525091.1:234485..234465:+ | 420.77 | 246.32 | 956.98 | 20,202.68 |
asi-miR-1890 | UGAAAUCUUUGAUUAGGUCUGG | 22 | KE524970.1:186795..186816:+ | 1025.26 | 245.15 | 900.89 | 499.34 |
asi-miR-137 | UAUUGCUUGAGAAUACACGUAG | 22 | KE525275.1:771433..771454:− | 745.69 | 222.97 | 334.05 | 453.38 |
asi-miR-932-5p | UCAAUUCCGUAGUGCAUUGCAG | 22 | KE525415.1:21588..321609:− | 22.67 | 221.80 | 552.42 | 627.10 |
asi-miR-965 | UAAGCGUAUAGCUUUUCCCAUU | 22 | KE524970.1:286640..286719:+ | 137.60 | 171.60 | 72.74 | 103.61 |
asi-miR-276-5p | AGCGAGGUAUAGAGUUCCUAC | 21 | KE525297.1:1458630..1458610:+ | 41.75 | 128.41 | 118.69 | 180.73 |
asi-miR-1000 | AUAUUGUCCUGUCACAGCAGU | 21 | KE524842.1:961875..961895:+ | 912.06 | 126.08 | 133.27 | 148.01 |
asi-miR-13a-3p | UAUCACAGCCAUUUUGAUGAGU | 22 | KE525350.1:1782958..1782938:+ | 33.62 | 74.71 | 41.04 | 49.86 |
asi-miR-2944a-5p | GAAGGAACUUCUGCUGUGAUCUGA | 24 | KE525343.1:1543524..1543501:+ | 42,259.28 | 64.21 | 17.75 | 61.54 |
asi-miR-285 | UAGCACCAUUCGAAAUCAGUAC | 22 | KE525331.1:1654228..1654249:+ | 7.97 | 52.53 | 617.71 | 701.10 |
asi-miR-79-3p | UAAAGCUAGAUUACCAAAGCAU | 22 | KE525317.1:663064..663043:+ | 65.52 | 51.36 | 30.90 | 22.59 |
asi-miR-210 | CUUGUGCGUGUGACAACGG | 19 | KE525157.1:631346..631328:+ | 39.25 | 49.03 | 119.48 | 330.30 |
asi-miR-124 | UAAGGCACGCGGUGAAUGC | 19 | KE525231.1:190116..190134:− | 91.16 | 47.86 | 43.58 | 50.64 |
asi-miR-988-5p | GUGUGCUUUGUGACAAUGAGA | 21 | KE525307.1:126115..126135:+ | 75.21 | 40.86 | 36.61 | 70.11 |
asi-miR-307 | CACAACCUCCUUGAGUGAGCGA | 22 | KE525347.1:4066618..4066639:− | 11.88 | 29.18 | 32.64 | 41.29 |
asi-miR-252-3p | CUGCUGCCCAAGUGCUUAUCG | 21 | KE525267.1:347039..347059:− | 6.25 | 22.18 | 10.30 | 11.69 |
asi-miR-988-3p | CCCCUUGUUGCAAACCUCACGC | 22 | KE525307.1:126074..106095:+ | 17.98 | 18.68 | 12.36 | 24.15 |
asi-miR-33 | GUGCAUUGUAGUUGCAUUGCA | 21 | KE524855.1:1060739..1060719:− | 0.47 | 8.17 | 1.43 | 2.34 |
asi-miR-2944b-5p | GAAGGAACUCCCGGUGUGAUAUA | 23 | KE525324.1:712584..712563+ | 2128.40 | 5.84 | 2.38 | 7.01 |
asi-miR-79-5p | GCUUUGGCGCUUUAGCUGUAUGA | 23 | KE525317.1:663023..663001:+ | 16.89 | 5.84 | 1.43 | 6.23 |
asi-miR-929 | AUUGACUCUAGUAGGGAGUCC | 21 | KE524723.1:863..843:+ | 11.26 | 5.84 | 11.09 | 12.46 |
asi-miR-277-5p | CGUGUCAGAAGUGCAUUUACA | 21 | KE524932.1:25192..25212:− | 4.53 | 4.67 | 97.93 | 52.97 |
asi-miR-286b | UGACUAGACCGAACACUCGUAUCCC | 25 | KE525324.1:712419..712396:+ | 10,890.76 | 2.33 | 10.30 | 24.93 |
asi-miR-2944b-3p | UAUCACAGCAGUAGUUACCUGA | 22 | KE525324.1:712629..712610 | 124.93 | 2.33 | 0.16 | 0.78 |
asi-miR-193 | UACUGGCCUACUAAGUCCCAAC | 22 | KE524784.1:191638..191659:− | 39.72 | 2.33 | 251.96 | 24.15 |
asi-miR-219 | UGAUUGUCCAAACGCAAUUCUUG | 23 | KE525339.1:431460..431438:+ | 2.50 | 2.33 | 5.23 | 0.78 |
asi-miR-286a | UGACUAGACCGAACACUCGCGUCCU | 25 | KE525343.1:1543040..1543016:+ | 227.04 | 1.17 | 0.32 | 0.00 |
asi-miR-263b-3p | UGGAUCUUUUCGUGCCAUCGU | 21 | KE524589.1:97592..97572:+ | 9.54 | 1.17 | 3.01 | 1.56 |
asi-miR-308-3p | AAUCACAGGAGUAUACUG | 18 | KE524853.1:228639..228622:+ | 2.19 | 1.17 | 1.27 | 3.12 |
asi-miR-4968-3p | CAGCAACAGCAGCAGCAGCAGA | 22 | KE525077.1:54358:54337:+ | 0.31 | 1.17 | 0.00 | 0.00 |
asi-miR-275-5p | CGCGCUAAGCAGGAACCGAGAC | 22 | KE524841.1:27320..27337:− | 0.00 | 1.17 | 0.63 | 0.00 |
asi-miR-2943 | UUAAGUAGGCACUUGCAGGCAAA | 23 | KE525350.1:266997..266975+ | 5825.57 | 0.00 | 0.00 | 0.00 |
asi-miR-2944a-3p | UAUCACAGUAGUUGUACUUUAA | 22 | KE525324.1:712814..712793:+ | 225.32 | 0.00 | 0.16 | 0.78 |
asi-miR-309a | UCACUGGGCAAAGUUUGUCGC | 21 | KE525324.1:713000..712980:+ | 65.67 | 0.00 | 0.00 | 3.90 |
asi-miR-315-3p | CUUUCGAGCAGUAAUCAAAGUC | 22 | KE524190.1:262648..262669:− | 2.19 | 0.00 | 0.00 | 0.78 |
asi-miR-1891 | UGAGGAGUUAAUUUGCGUGUUU | 22 | KE525346.1:7507..7486:+ | 1.41 | 0.00 | 0.95 | 2434.39 |
asi-miR-980-3p | UAGCUGCCUAGUGAAGGGC | 19 | KE524190.1:422320..422338:+ | 1.25 | 0.00 | 2.54 | 10.13 |
asi-miR-13-5p | UCGUAAAAAUGGUUGUGCUGUG | 22 | KE524905.1:90055..90038:+ | 0.63 | 0.00 | 0.63 | 0.00 |
asi-miR-iab-4-3p | CGGUAUACCUUCAGUAUACGUAAC | 24 | KE525233.1:295549..295572:− | 0.63 | 0.00 | 0.00 | 0.00 |
asi-miR-2491-3p | CAACAACAGCAGCAGCAA | 18 | KL646034.1:671..688:+ | 0.00 | 0.00 | 0.16 | 0.00 |