Fig. 4
From: INAVA promotes aggressiveness of papillary thyroid cancer by upregulating MMP9 expression
The effect of INAVA on PTC cells is mediated by MMP9. a Transcriptome resequencing was performed on TPC1-Vector and TPC1-INAVA cells and mRNA expression of the indicated genes related to invasion/metastasis is shown. b Altered cellular mRNA levels of MMP9 in the indicated cells were detected by real time RT-PCR assays. GAPDH served as a house keeping gene control. c WB assays were conducted to analyze the protein levels of MMP9 in response to deregulated INAVA expression in TPC1 and K1 cells. α-Tubulin acted as a loading control. d MMP9 activities in indicated cells were analyzed using gelatin zymography. Knockdown of MMP9 in INAVA overexpressing cells (e), attenuated phenotypic characteristics induced by INAVA overexpression (f). For b, f, data represents mean ± SD of 3 independent experiments in the bar graphs. *P < 0.05