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Fig. 6 | Cell & Bioscience

Fig. 6

From: Enhanced calcium ion mobilization in osteoblasts on amino group containing plasma polymer nanolayer

Fig. 6

Calcium imaging in vital fluo-3 loaded human osteoblasts—cell line (MG-63) and primary osteoblasts (HOB). Changes in fluorescence intensities of the cells were detected using confocal laser scanning microscopy (LSM780, Carl Zeiss, ZEN-software). a The representative fluorescence images, after ATP stimulation (240 s = 120th cycle), show the Ca2+ increase on Ti + PPAAm surfaces compared with bare Ti in MG-63 as well as HOB cells. (Scale bar MG-63: 20 µm, HOB: 50 µm). b Intracellular Ca2+ dynamics in vital primary osteoblasts (HOB) over 480 s (= 240 cycles) every 2 s on Ti + PPAAm compared with Ti. Note the significantly enhanced intracellular calcium ion signal in HOB cells after ATP stimulus (180 s) on Ti + PPAAm. c Intracellular Ca2+ signal of MG-63 cells over time on Ti + PPAAm compared with Ti. Significant differences in the intracellular Ca2+ dynamic of MG-63 after ATP stimulation was detectable (see explanation in b). MG-63 and HOB established the same cellular reactivity in calcium signaling on Ti + PPAAm and bare Ti. (MG-63 = 3, and HOB = 5 independent approaches each for 10 defined areas of 10 cells per time point, 240 cycles, polygon line as mean ± s.e.m., multiple t-test, *p < 0.05)

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