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Fig. 4 | Cell & Bioscience

Fig. 4

From: Enhanced calcium ion mobilization in osteoblasts on amino group containing plasma polymer nanolayer

Fig. 4

Fluorescence intensity of the Ca2+ mobilization in osteoblasts over a time series using confocal laser scanning microscopy (LSM780, Carl Zeiss) and the software ZEN2 (blue edition, Carl Zeiss) in the mode “mean region of interest (ROI)”. a In the first fluorescence image of the time series, the defined areas in 10 cells (one area per cell) are fixed. b Graphical representation of the mean fluorescence intensity (MFI) of the 10 defined areas over the entire time series

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