Fig. 4
From: Enhanced calcium ion mobilization in osteoblasts on amino group containing plasma polymer nanolayer
Fluorescence intensity of the Ca2+ mobilization in osteoblasts over a time series using confocal laser scanning microscopy (LSM780, Carl Zeiss) and the software ZEN2 (blue edition, Carl Zeiss) in the mode “mean region of interest (ROI)”. a In the first fluorescence image of the time series, the defined areas in 10 cells (one area per cell) are fixed. b Graphical representation of the mean fluorescence intensity (MFI) of the 10 defined areas over the entire time series