Fig. 5

The interaction between miR-141-3p and Notch-1 in pulmonary epithelial cells. a The pulmonary epithelial cell line MLE-12 was transfected with miR-141-3p mimic or its control (pre-NC), and b the mRNA and c protein expressions of Notch-1 and Hesr1 were then determined. d MLE-12 cells were transfected with miR-141-3p inhibitor or its control (NC) and then treated by PQ. The expressions of Notch-1 and Hesr1 protein were detected using western blot. e MLE-12 cells were divided into six groups, which were received diverse treatment: MLE-12 cells cultured in normal condition; MLE-12 cells treated by PQ; MLE-12 cells transfected with miR-141-3p inhibitor or its control (NC) and then treated by PQ. MLE-12 cells co-transfected with miR-141-3p inhibitor and si-Notch-1 or si-control and then treated by PQ. The expressions of Nrf2, Hmox1 and Txnrd1 protein were detected using western blot. **P < 0.01 vs. pre-NC