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Fig. 6 | Cell & Bioscience

Fig. 6

From: LncRNA KCNQ1OT1 promotes osteogenic differentiation to relieve osteolysis via Wnt/β-catenin activation

Fig. 6

Effect of KCNQ1OT1 on β-catenin in mMSCs differentiation. a The mRNA and protein expression levels of β-catenin in mMSCs transfected with pcDNA-KCNQ1OT1, sh-NC or pcDNA-KCNQ1OT1 along with siRNA-β-catenin before the addition of PMMA particles. b ALP activity in mMSCs transfected with pcDNA-KCNQ1OT1, sh-NC or pcDNA-KCNQ1OT1 along with siRNA-β-catenin before the addition of PMMA particles. c The mRNA expression levels of Runx2, Osterix and OCN in mMSCs transfected with pcDNA-KCNQ1OT1, sh-NC or pcDNA-KCNQ1OT1 and siRNA-β-catenin before the addition of PMMA particles. d Osteoblastic differentiation in mMSCs transfected with pcDNA-KCNQ1OT1, sh-NC or pcDNA-KCNQ1OT1 along with siRNA-β-catenin before the addition of PMMA particles. e The protein and mRNA levels of β-catenin in KCNQ1OT1 pulled down pellet using IgG antibody as negative control by RNA pull-down assay. *P < 0.05 compared with NC. *P < 0.05 compared with control group. &P < 0.05 compared with PMMA + pcDNA group. #P < 0.05 compared with PMMA + pcDNA-KCNQ1OT1 group

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