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Fig. 4 | Cell & Bioscience

Fig. 4

From: Dimerized translationally controlled tumor protein increases interleukin-8 expression through MAPK and NF-κB pathways in a human bronchial epithelial cell line

Fig. 4

Inhibition of NF-κB blocks IL-8 induction by dTCTP. a BEAS-2B cells were pre-incubated with the IκB kinase inhibitor, BAY 11-7082, at the indicated doses for 30 min and then treated with 10 μg/ml of dTCTP for an additional 16–20 h. The resulting IL-8 in the supernatant were analyzed by ELISA. Values represent mean ± SEM (n = 3). *p < 0.05, ***p < 0.001, significantly different from control group. b BEAS-2B cells were transfected with control siRNA or p65 siRNA (20 nM) for 24 h as described in the Methods section. After 16–20 h of treatment with or without 10 μg/ml of dTCTP, cell lysates (5 μg) were immunoblotted and probed with p65 or β-actin antibody (upper panel). The supernatant was harvested and the IL-8 content was measured using ELISA (lower panel). Values represent mean ± SEM (n = 3). ns not significant from untreated group. ***p < 0.001, significantly different from untreated group

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