Fig. 3

CCL18 is a potential target gene of miR-622. a The predicted result from targetscan. b MiR-622 mRNA levels in OS-RC-2 and ACHN cells. OS-RC-2 and ACHN cells were transfected with miR-622 mimics for 48 h and RNA was extracted for RT-PCR. c MiR-622 mRNA levels OS-RC-2 and ACHN cells. OS-RC-2 and ACHN cells were transfected with miR-622 inhibitors for 48 h and RNA was extracted for RT-PCR. d CCL18 protein levels in OS-RC-2 and ACHN cells with miR-622 mimics transfection were texted by ELISA. e CCL18 protein levels in OS-RC-2 and ACHN cells with miR-622 inhibitors transfection were texted by ELISA. f Luciferase activity in 293T cells was down-regulated significantly when the cells were co-transfected with miR-622 mimics and wide type of CCL18 3′UTR. g Luciferase activity in 293T cells was down-regulated significantly when the cells were co-transfected with miR-622 inhibitors and wide type of CCL18 3′UTR. **P < 0.01