Fig. 4

MiR-210-3p modulated MDR-1 expressions of RCC cells via ABCC1. a Caki-2 cells were transfected with si-ABCC1 or si-control, the knockdown efficiency of ABCC1 was detected using qRT-PCR and Western blot assays. Caki-2 cells were transfected with miR-210-3p inhibitor or miR-210-3p inhibitor + si-ABCC1, and then the levels of MDR-1 were assessed by Western blot assays. b Caki-2/DOX and Caki-2/VBL cells were transfected with pcDNA-ABCC1 or pcDNA, the overexpression efficiency of ABCC1 was detected using qRT-PCR and Western blot assays. Caki-2/DOX and Caki-2/VBL cells were transfected with miR-210-3p mimic or miR-210-3p mimic + pcDNA-ABCC1, and then the levels of MDR-1 were assessed by Western blot assays. *P < 0.05 vs. si-control or pcDNA