Fig. 3
Exogenous H2S increased cell viability and decreased apoptotic rate in the aged H9C2 cells. a Cell viability was measured by CCK-8 kit. All data are mean ± S.E.M. of 8 determinations. b Detection of nuclear morphology in apoptotic cells by Hoechst 33342 staining. Apoptotic cells were identified as cells with condensed, disrupted nuclei. The cells of arrow indicate the apoptotic cells (400× magnification). Scale bar = 100 μm. Apoptotic cells in at least five random fields were counted. c Apoptosis analyzed by flow cytometry. The apoptotic rate = early apoptotic rate + late apoptotic rate. All Data were from four independent experiments. **p < 0.01 vs. control group; #p < 0.05 vs. H/R group; &&p < 0.01 vs. PC group; $p < 0.05 vs. H/R + NaHS group