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Fig. 2 | Cell & Bioscience

Fig. 2

From: Exogenous H2S restores ischemic post-conditioning-induced cardioprotection through inhibiting endoplasmic reticulum stress in the aged cardiomyocytes

Fig. 2

Low dose of H2O2 induced senescence in the H9C2 cells without causing apoptosis. a The AGEs content. The different concentrations (0, 5, 10, 30, 50, 80, 100 μM) H2O2 was added to the H9C2 cells in the culture cluster for 2 h and subsequently cultured for 3 days. All data were from eight independent experiments. *p < 0.05, **p < 0.01 vs. control group. b The caspase-3 activity. Caspase-3 activity, measured as luminescence, in the H9C2 cells 3 days after treatment with H2O2 (30, 50, 80, 100 μM) for 2 h. All data were from four independent experiments. **p < 0.01 vs. control group. c The number of SA β-gal-positive H9C2 cells. Phase-contrast images showing morphologic changes and stained cells. (SA-β-gal positive cells; Blue, magnification ×400). Scale bar = 50 μm. The aged H9C2 cells in at least five random fields were counted. **p < 0.01 vs. control group. d The expression of cyclin D1 and p21Cip/WAF−1. The intensity of each band was quantified by densitometry, and data were normalized to the GAPDH signal. All data were from four independent experiments. *p < 0.05 vs. control group

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