Fig. 6

miR-124 overexpression promoted proliferation and neural differentiation in NSCs by targeting DLL4. NSCs were transfected with miR-124, miR-control, miR-124 + vector, or miR-124 + pcDNA-DLL4. a Western blot analysis of DLL4 protein level in transfected NSCs. b MTT assay was performed to detect cell viability at 0, 24, 48, and 72 h in transfected NSCs. The mRNA (c) and protein (d) levels of ki-67 in transfected NSCs were determined by qRT-PCR and western blot. e The mRNA expression of Nestin in transfected NSCs was examined by qRT-PCR. f The number of neurosphere after transfection (diameter > 50 mm) and the averaged neurosphere diameter in different groups were analyzed. The mRNA and protein levels of β-tubulin-III (g, i) and GFAP (h, j) in transfected NSCs. *P < 0.05, n = 3