Fig. 1

NSCs could self-proliferate and differentiate into neuron and astrocyte. a Photomicrographs of representative neurospheres are displayed. b Purified NSCs are subjected to immunocytochemical staining with Nestin. c Nuclear staining of differentiated cells from NSCs with DAPI. d Immunocytochemical staining of purified neurons with β-tubulin-III (×400). e Immunocytochemical staining of purified protoplasmic astrocytes with GFAP (×400). n = 3