Fig. 3

The methylation of Gadd45β promoter in HCC tissues and liver cancer cell lines. The status of methylation of Gadd45β promoter in HCC tissues and cells were studied by methylation-specific PCR (MSP) assay. a The HCC tumor tissues showed the obvious methylation status; however, the peri-tumor samples indicated strong unmethylated PCR products. b The determination of status of Gadd45β promoter methylation in liver cancer cell lines and normal liver cell. Methylated PCR products were detected in SMMC-7721 and Hep-3B cells; however, normal liver cells L-02 indicated unmethylated PCR products. “M” means methylation, “U” means un-methylation. The amplified methylation bands size is 137 bp; The unmethylated bands size is 128 bp. Negative control: pure water