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Fig. 2 | Cell & Bioscience

Fig. 2

From: Methylation mediated Gadd45β enhanced the chemosensitivity of hepatocellular carcinoma by inhibiting the stemness of liver cancer cells

Fig. 2

Gadd45β overexpression inhibited the stemness of liver cancer cells, enhancing apoptosis of cancer cells. a After the transfection of Gadd45β-expressing virus, western blot was performed to examine the Gadd45β expression. GAPDH was used as an internal reference. b The Gadd45β transfected SMMC-7721 and Hep-3B cells indicated the decreased colony formation. c Quantification of the clone numbers of cancer cells. Data are presented as the mean ± SD (** < 0.01). d, e PCR assay was used to detect the stemness makers’ expression in Gadd45β transfected cells. The results showed that transfection of Gadd45β resulted in the downregulation of SOX 9 and OCT4 mRNA expression in SMMC-7721 and Hep-3B cells. GAPDH was used as an internal reference (*P < 0.05; **P < 0.01; ***P < 0.001). f Western blot assay was then used to detect the level of SOX9 and OCT4 protein of cells. Gadd45β transfected SMMC-7721 and Hep-3B cells indicated a lower SOX9 and OCT4 protein level than vector control group. The protein levels were normalized by a comparison with the GAPDH level. g Cell viability assay showed that Gadd45β overexpression enhanced the cisplatin-induced apoptosis of SMMC-7721 and Hep-3B cells

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