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Fig. 2 | Cell & Bioscience

Fig. 2

From: Cloning, expression and identification of KTX-Sp4, a selective Kv1.3 peptidic blocker from Scorpiops pococki

Fig. 2

The expression, purification and identification of peptide KTX-Sp4. a Tricine/SDS-PAGE analysis of the purification of KTX-Sp4 peptide. M, molecular mass markers; Lane 1, proteins from non-induced coli Rosetta (DE3) cells; lane 2, proteins from induced coli Rosetta (DE3) cell containing pGEX-4T-1-KTX-Sp4 by IPTG; lane 3, purified GST fusion protein after affinity chromatography and desalting; lane 4, fusion protein cleaved by enterokinase; lane 5, purified KTX-Sp4 by reversed phase HPLC. b Purification of KTX-Sp4 by HPLC on a C18 column. c Mass spectrum of KTX-Sp4 peptide measured by MALDI-TOF–MS. Measured value is 4545.3 Da, and the calculated one is 4547.3 Da

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