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Fig. 5 | Cell & Bioscience

Fig. 5

From: 14-3-3ζ loss leads to neonatal lethality by microRNA-126 downregulation-mediated developmental defects in lung vasculature

Fig. 5

14-3-3ζ mediates miR-126 transcription by modulating Ets2 activity. a Detection of 14-3-3ζ, phosphor-Erk 1/2 (Thr202/Tyr204), total Erk1/2 and β-actin by western blotting in mouse endothelial cells expressing either a scrambled shRNA or shRNA targeting 14-3-3ζ. b, c ChIP analysis of Ets2 and Ets1 binding to the miR-126 promoter in mouse endothelial cells expressing either a scrambled shRNA or shRNA targeting 14-3-3ζ, ± SD (n = 3). d Analysis of phosho-Erk (Thr202/Tyr204) and Erk1/2 in mouse endothelial cells treated with either DMSO or the ERK1/2 inhibitor AZD6244 by western blotting. e qRT-PCR analysis of miR-126 expression relative to PECAM1 in mouse endothelial cells treated with either DMSO or AZD6244, ± SD (n = 3). f ChIP analysis of Ets2 binding to the miR-126 promoter in mouse endothelial cells treated with either DMSO or AZD6244. ± SD. g ChIP analysis of Ets1 binding to the miR-126 promoter in mouse endothelial cells treated with either DMSO or AZD6244. ± SD (n = 3)

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