Fig. 1From: Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cellsRegulation of IFNα, IFNβ and IFNε by various cytokines in A2EN cells and primary cervical cells. A2EN or primary cervical epithelial cells were treated with stated cytokines at 1 or 10 ng/ml for 4 or 24 h. Total RNAs were prepared and gene expression of IFN⍺, IFNβ and IFNε was measured by real-time RT-PCR. Induction of each gene (as fold-change relative to untreated control) was calculated using the ΔΔCT method as described in “Materials and methods”. The value of 1 indicates the level of gene expression of untreated control cells. Data for TNFα and IL-1β are mean ± SD of four independent experiments in A2EN cells (a) and of experiments from primary cervical cells from different donors (b). Average of fold-changes of other cytokines-treated samples relative to untreated samples from four different experiments were summarized in c. Numbers in bold represents significant changes (*p < 0.05). ⍭p=0.057–0.07Back to article page