Fig. 7

Immunostaining of GFAP shows blunted recruitment of GFAP+ astrocytes in CD1 mice relative to C57BL/6 mice after various length of cuprizone exposure. a Representative images of GFAP+ astrocytes at the midline of the corpus callosum. Scale bar, 30 μm. b Quantification of cell density counts. CD1 mice and C57BL/6 mice show comparable numbers of GFAP and to-pro-3 double positive astrocytes under control conditions, and significant differences between strains were observed at 6 and 7 weeks of treatment (asterisks above brackets indicate significant interaction between strain and cuprizone in two-way ANOVA analysis, P < 0.01). Asterisks above the bars indicate significant differences from controls (t-test, *P < 0.05; **P < 0.01). Error bars show SEM. N numbers are shown on individual bars