Fig. 5

Immunostaining of NG2 shows blunted recruitment of NG2+ oligodendrocyte progenitor cells (OPCs) in CD1 mice relative to C57BL/6 mice at different length of cuprizone exposure. a Representative images of NG+ OPCs at the midline of the corpus callosum. Scale bar, 30 μm. b Quantification of cell density counts. CD1 mice and C57BL/6 mice show comparable numbers of NG2 and to-pro-3 double positive OPCs under control conditions, and significant differences between strains were observed at 4 and 6 weeks of treatment (asterisks above brackets indicate significant interaction between strain and cuprizone in two-way ANOVA analysis, *P < 0.05; **P < 0.01). At 7 weeks of treatment, the numbers of OPCs in the two strains appear to be similar. Asterisks above the bars indicate significantly different from respective controls (t-test, *P < 0.05; **P < 0.01). Error bars show SEM. N numbers are indicated on individual bars