Fig. 2

LFB-PAS staining reveals relatively mild demyelination in CD1 mice in comparison to C57BL/6 mice. a Representative images of the LFP-PAS stained corpus callosum in C57BL/6 mice and CD1 mice. Myelin shows up as blue stain and the demyelinated area shows up as pink. Note C57BL/6 sections show more pink and less blue compared to CD1 mice. Scale bar, 100 µm. b Semi-quantification of demyelination by blind scoring (3 = intact and 0 = complete demyelination). Data is shown as group averages. N = 4 and 7 at 4 weeks for C57 and CD1, respectively. N = 5 and 5 for 5 weeks, N = 4 and 5 for 6 weeks and N = 5 and 7 for 7 weeks. Significant differences between strains were observed at 4 to 6 weeks of cuprizone treatment (student’s t-test). **indicates P < 0.001, *indicates P < 0.05