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Fig. 6 | Cell & Bioscience

Fig. 6

From: Differential impact of various reactive oxygen species (ROS) on HIF-1α/p53 direct interaction in SK-N-MC neuroblastoma cells

Fig. 6

Direct interaction between HIF1-α and p53 under oxidant and imitated hypoxic condition by immunoprecipitation of p53 along with HIF1-α. SK-NM-C cells were treated with either 100 μM H2O2, 12 μM menadione or 100 μM CoCl2 for 24 h. Following cells lysis, immunoprecipitation was performed using p53 antibody and the immune detection was achieved using either HIF-1α (top) or p53 (bottom) antibodies. (IP-) refers to samples processed without incubation with p53 antibody

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