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Fig. 5 | Cell & Bioscience

Fig. 5

From: Differential impact of various reactive oxygen species (ROS) on HIF-1α/p53 direct interaction in SK-N-MC neuroblastoma cells

Fig. 5

p53 activation by manadione, H2O2 and CoCl2. SK-N-MC cells were treated separately with 100 μM H2O2, 12 μM menadione and/or 100 μM CoCl2 for 24 h. P53 expression was analyzed by Western blot technique. The Intensity of each band was examined with densitometry analysis and values were normalized with respect to B-tubulin and were shown relative to the control cells. The histogram values represent the mean ± SD for three independent experiments; asterisk significantly different from untreated control cells (p < 0.05), one representative western blot is shown

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