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Fig. 4 | Cell & Bioscience

Fig. 4

From: Differential impact of various reactive oxygen species (ROS) on HIF-1α/p53 direct interaction in SK-N-MC neuroblastoma cells

Fig. 4

HIF1-α stabilization by manadione, H2O2 and CoCl2. SK-N-MC cells were treated with 100 μM H2O2, 12 μM menadione and 100 μM CoCl2 for 24 h. Protein expression was analyzed by Western blot technique. The intensity of each band was examined using densitometry analyses and the values were normalized with respect to B-tubulin and are shown relative to control. The histogram values represent the mean ± SD for 3 independent experiments. Asterisk significantly different from control cells (p < 0.05), one prototype of western blot is shown

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