Fig. 3
Evaluating apoptosis in SK-N-MC cells. a Cells were treated with 100 μM H2O2, 12 μM menadione and 100 μM CoCl2 for 24 h and then cells were visualized with Ethidium bromide/acridine orange dual-staining method. b The extent of apoptosis was determined based on counting apoptic cells in ten randomly chosen region by fluorescence microscope. c Caspase3 generation, as an apoptotic marker under oxidative stress condition, was analyzed using western blot technique. Asterisk significantly different from untreated control cells (p < 0.05)