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Fig. 3 | Cell & Bioscience

Fig. 3

From: Differential impact of various reactive oxygen species (ROS) on HIF-1α/p53 direct interaction in SK-N-MC neuroblastoma cells

Fig. 3

Evaluating apoptosis in SK-N-MC cells. a Cells were treated with 100 μM H2O2, 12 μM menadione and 100 μM CoCl2 for 24 h and then cells were visualized with Ethidium bromide/acridine orange dual-staining method. b The extent of apoptosis was determined based on counting apoptic cells in ten randomly chosen region by fluorescence microscope. c Caspase3 generation, as an apoptotic marker under oxidative stress condition, was analyzed using western blot technique. Asterisk significantly different from untreated control cells (p < 0.05)

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