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Fig. 5 | Cell & Bioscience

Fig. 5

From: Genome editing of the HIV co-receptors CCR5 and CXCR4 by CRISPR-Cas9 protects CD4+ T cells from HIV-1 infection

Fig. 5

lenti-X4R5-Cas9 modified primary CD4+ T cell resists HIV challenge. a T7E1 analysis of CXCR4 and CCR5 disruption. b Deep sequencing analysis of typical NHEJ (indels) of related targets. c lenti-X4R5-Cas9 modified CD4+ T cell challenged with HIV-1NL4-3 or HIV-1YU-2. d lenti-X4R5-Cas9 modified CD4+ T cell exposed to dual-tropic HIV-1 variants (NL4-3 & YU-2, 1:1). The CCR5, CXCR4-1, CXCR4-2 represent single disruption of CCR5 or CXCR4, which use the same corresponding gRNAs used in lenti-X4R5-Cas9-#1 or #2. The data shown were the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; t test

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