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Table 1 Comparative quantification of protein bound along column performing

From: Investigation of host–pathogen interaction between Burkholderia pseudomallei and autophagy-related protein LC3 using hydrophobic chromatography-based technique

Condition of column Initial protein (µg ± SD)a Flow-through (µg ± SD)a Bound protein (µg ± SD)a % of bound proteinb
Column set up
 Recombinant LC3 binding control 50 12.67 ± 0.54 37.33 ± 0.54 74.65 ± 0.96
 Empty pET17b competent binding control 50 25.58 ± 0.27 24.42 ± 0.27 49.24 ± 0.01
Bacterial protein (0.5 µg/µl) applying step
 B. pseudomallei WT 36.54 ± 0.54 23.08 ± 1.63 13.46 ± 1.09 36.87 ± 3.53
 B. pseudomallei rpoS mutant 37.12 ± 1.36 24.42 ± 0.27 12.69 ± 1.63 34.14 ± 3.15
 Negative controlc 24.42 ± 0.27 19.42 ± 1.36 5.00 ± 1.09 20.50 ± 4.68
  1. aData was obtained from independent experimental replicate
  2. b% of bound protein was calculated from bound protein (μg) × 100/initial protein existed in column (μg)
  3. cNegative control represent to a column condition containing crude proteins without LC3 expression bait for protein of B. pseudomallei wild type (WT) strain