Fig. 3

RpoS-dependent promoter prediction and quantification. a 150 nucleic acids upstream of complementary strand of gene encoding ABC transporter periplasmic substrate-binding protein (bpsl2203) was predicted RpoS-dependent promoter. b 10 promoter element was predicted by HMM analysis. The first line is representing to HMM consensus of RpoS promoter in B. pseudomallei. The second line is showing the letter perfectly matches to the consensus sequence of RpoS promoter, and the third line is the query sequence. c Sequence logo of RpoS-dependent promoter at −10 to −4 positions. d bpsl2203 gene was relatively quantified comparing between wild type and rpoS mutant. Data were normalized by 23S rRNA expression level. Asterisk indicates to significant difference between these strains (p ≤ 0.01)