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Fig. 1 | Cell & Bioscience

Fig. 1

From: Investigation of host–pathogen interaction between Burkholderia pseudomallei and autophagy-related protein LC3 using hydrophobic chromatography-based technique

Fig. 1

Burkholderia pseudomallei PP844 infection in U937 cell lines. a Western blot analysis verifying expression level of an autophagy-related protein LC3 in both isoforms; LC3-I and LC3-II, in U937 infected B. pseudomallei after 24 h of post infection at MOI20. Lane A represents to mock infection. Lane B and C represent to wild type PP844 and rpoS mutant infection, respectively. b Colocalization detection of B. pseudomallei (Alexa 488) and LC3 (Alexa 594). c Colocalization detection of B. pseudomallei (Alexa 488) and a lysosomal marker, cathepsin D (Alexa 594). d Pearson’s correlation coefficient (PCC) of colocalization from fluorescence intensity of bacteria with LC3 and bacteria with cathepsin D. A statistically significant difference between two processes of autophagy was determined using Mann–Whitney Rank Sum Test (p ≤ 0.001)

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