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Fig. 3 | Cell & Bioscience

Fig. 3

From: A human plasma derived supplement preserves function of human vascular cells in absence of fetal bovine serum

Fig. 3

Analysis of cell structure by immunofluorescence. In terms of cell structure, CD31 was stained for endothelial cells, actin α smooth muscle for smooth muscle cells and collagen I for fibroblasts. Specific proteins are stained in green and nuclei in blue. Cellular orientation distribution was measured using Fiji’s plugin “Orientation J Distribution”. The Gaussian distribution of cellular orientations was centered on 0 and all values were divided by each case’s maximum value in order to get a normalized orientation value between 0 and 1. The mean dispersion percentage was calculated as the angle range that contained 80% of the orientations, divided by 180°

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